6TE3
Crystal Structure of full-length Human Lysyl Hydroxylase LH3 - Cocrystal with Fe2+, Mn2+, UDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-11 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 96.995, 100.035, 225.208 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.840 - 2.300 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fxm |
RMSD bond length | 0.004 |
RMSD bond angle | 0.618 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.840 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.118 | 1.476 |
Rpim | 0.086 | 1.107 |
Number of reflections | 48288 | 4455 |
<I/σ(I)> | 8.4 | 0.9 |
Completeness [%] | 98.7 | 99.7 |
Redundancy | 4.8 | 4.8 |
CC(1/2) | 0.997 | 0.512 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.8 | 277 | 600 mM sodium formate, 12% PGA-LM, 100 mM HEPES/NaOH, 500 mM FeCl2, 500 mM MnCl2, 1 mM UDP |