6TDB

Neuropilin2-b1 domain in a complex with the C-terminal VEGFB167 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.977
Spacegroup name	C 1 2 1
Unit cell lengths	107.359, 140.122, 74.357
Unit cell angles	90.00, 132.97, 90.00

Refinement procedure

Resolution	70.060 - 2.450
R-factor	0.1859
Rwork	0.183
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rn5
RMSD bond length	0.004
RMSD bond angle	0.691
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.060	70.060	2.580
High resolution limit [Å]	2.450	7.750	2.450
Number of reflections	29170	928	4278
<I/σ(I)>	10.6
Completeness [%]	98.9
Redundancy	3.4
CC(1/2)	0.996	0.997	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	N2b1-apo 0.05 M KCl, 0.01 M MgCl2 and 15 % (w/v) PEG600 N2b1/VEGFB167 0.1 M HEPES pH 7.5, 10 % (w/v) PEG4000, 20 % (w/v) isopropanol