6TCJ
Crystal structure of the BCL6 BTB domain in complex with a hybrid BTB-binding (HBP) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.657, 47.300, 76.370 |
| Unit cell angles | 90.00, 94.34, 90.00 |
Refinement procedure
| Resolution | 40.210 - 2.130 |
| R-factor | 0.2022 |
| Rwork | 0.199 |
| R-free | 0.24950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r2b |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.271 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.210 | 2.190 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.183 | |
| Rpim | 0.172 | |
| Number of reflections | 15991 | 1171 |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 0.981 | 0.386 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 9.9% (v/v) MPD, 2.64% (w/v), PEG 8000 0.5 M NaCl, 0.1M Tris pH 8.5 |
