6TCI

The crystal structure of SleB N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	P 41 21 2
Unit cell lengths	43.330, 43.330, 72.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.640 - 1.470
R-factor	0.1738
Rwork	0.170
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lpq
RMSD bond length	0.004
RMSD bond angle	0.682
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15_3448)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.640	1.500
High resolution limit [Å]	1.470	1.470
Rmerge	0.079	1.586
Rmeas	0.079	0.160
Rpim	0.099	0.231
Number of reflections	12245	1128
<I/σ(I)>	17.4
Completeness [%]	99.5	94.39
Redundancy	8.8	6.7
CC(1/2)	0.886	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG 3350 containing 0.2 M potassium thiocyanate