6TC6

Crystal structure of MutM from Neisseria meningitidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9184
Spacegroup name	P 1 2 1
Unit cell lengths	66.378, 37.960, 110.303
Unit cell angles	90.00, 89.97, 90.00

Refinement procedure

Resolution	42.431 - 2.900
R-factor	0.2476
Rwork	0.245
R-free	0.29800
Structure solution method	MIR
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.431	2.952
High resolution limit [Å]	2.850	2.850
Number of reflections	19597	1162
<I/σ(I)>	9.23
Completeness [%]	96.4
Redundancy	29.5
CC(1/2)	0.844	0.207

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	100 mM Bicine pH 8.5; 20% (w/v) PEG 6000