6TBR

Glycosylated AA13 Lytic polysaccharide monooxygenase from Aspergillus oryzae in P1 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-07
Detector	MAR CCD 165 mm
Wavelength(s)	1.04
Spacegroup name	P 1
Unit cell lengths	46.060, 49.760, 57.100
Unit cell angles	109.37, 90.07, 95.46

Refinement procedure

Resolution	53.840 - 1.700
R-factor	0.1945
Rwork	0.192
R-free	0.24640
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.019
RMSD bond angle	1.853
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.840	1.740
High resolution limit [Å]	1.700	1.700
Rmeas	0.077	0.455
Number of reflections	48576	3521
<I/σ(I)>	2.87	9.04
Completeness [%]	92.6
Redundancy	1.68
CC(1/2)	0.994	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.01 M ZnCl2, 14% PEG6000, 0.1 M MES pH 6.0, 30% glycerol