6TA7

CRYSTAL STRUCTURE OF HUMAN G3BP1-NTF2 IN COMPLEX WITH HUMAN CAPRIN1-DERIVED SOLOMON MOTIF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-13
Detector	RAYONIX MX-225
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	81.558, 100.038, 113.345
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.540 - 1.930
R-factor	0.2081
Rwork	0.207
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fw5
RMSD bond length	0.007
RMSD bond angle	0.777
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.540	1.997
High resolution limit [Å]	1.928	1.928
Number of reflections	66520	4630
<I/σ(I)>	14.62	0.68
Completeness [%]	94.3	66.43
Redundancy	13.5
CC(1/2)	0.999	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M Sodium chloride, 0.1M Tris 8.0, 20% w/v PEG 4000 of the Proplex crystallization screen (Molecular Dimensions)