6T9X
Crystal structure of formate dehydrogenase FDH2 D222Q/Q223R mutant enzyme from Granulicella mallensis MP5ACTX8 in complex with NADP and Azide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9667 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.605, 69.189, 111.841 |
| Unit cell angles | 90.00, 93.78, 90.00 |
Refinement procedure
| Resolution | 45.751 - 2.200 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.20690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t8c |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.751 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.113 | 0.609 |
| Number of reflections | 430016 | 41605 |
| <I/σ(I)> | 11.3 | 2.4 |
| Completeness [%] | 98.4 | 98.83 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10 % w/v PEG 1000, 10%w/v PEG8000, 50 mM HEPES pH 7.0 |
