6T9Q

Crystal structure of the second, C-terminal repeat of the DNA-binding domain of human TImeless

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 65
Unit cell lengths	55.407, 55.407, 41.659
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.460 - 1.150
R-factor	0.1294
Rwork	0.128
R-free	0.15470
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.964
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.460	1.191
High resolution limit [Å]	1.150	1.150
Rmerge	0.138	0.487
Rmeas	0.154	0.650
Rpim	0.069	0.427
Number of reflections	25072	1920
<I/σ(I)>	6.82	1.36
Completeness [%]	96.3	74.84
Redundancy	4.2	1.6
CC(1/2)	0.975	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	200mM Sodium formate 18% PEG3350