6T8F
Crystal structure of mutant xylose isomerase (V270A/A273G) from Piromyces E2 grown in yeast, in complex with xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-08-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.598, 79.372, 91.982 |
| Unit cell angles | 115.46, 89.98, 117.13 |
Refinement procedure
| Resolution | 46.600 - 2.000 |
| R-factor | 0.1507 |
| Rwork | 0.149 |
| R-free | 0.18490 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5nh5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.549 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.355 |
| Rmeas | 0.111 | 0.479 |
| Rpim | 0.074 | 0.320 |
| Number of reflections | 111563 | 5325 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 95.3 | 92.1 |
| Redundancy | 2.2 | 2.2 |
| CC(1/2) | 0.992 | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14-17 % PEG3350, HEPES pH 7 |
