6T7O

X-ray structure of the C-terminal domain of S. aureus Hibernating Promoting Factor (CTD-SaHPF)

Replaces:  5OOX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	45.855, 45.855, 117.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.165 - 1.600
R-factor	0.162230671118
Rwork	0.161
R-free	0.18193
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k2t
RMSD bond length	0.013
RMSD bond angle	1.143
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.165	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.045	0.632
Number of reflections	17357	1692
<I/σ(I)>	28.12
Completeness [%]	99.9	99.7
Redundancy	13.1
CC(1/2)	0.999	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15M Citric acid pH 4.0, 24% PEG 6000