6T78

Structure of human Sox11 transcription factor in complex with a short DNA fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.999
Spacegroup name	P 61
Unit cell lengths	106.130, 106.130, 76.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.956 - 2.504
R-factor	0.2284
Rwork	0.227
R-free	0.25780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gt0
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.956	45.956	2.570
High resolution limit [Å]	2.500	11.200	2.500
Rmerge	0.126	0.036	2.786
Rmeas	0.129	0.037	2.858
Total number of observations	350700
Number of reflections	17066	206	1265
<I/σ(I)>	16.54	70.02	0.87
Completeness [%]	99.9	98.6	99.2
Redundancy	20.55	18.388	19.973
CC(1/2)	1.000	1.000	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	Sodium Acetate 100 mM pH 4.5, Calcium Acetate Hydrate 200 mM, 15-18 % PEG 400