6T76

PII-like protein CutA from Nostoc sp. PCC 7120 in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	62.133, 90.694, 93.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.850 - 1.900
Rwork	0.199
R-free	0.22580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v6h
RMSD bond length	0.017
RMSD bond angle	1.801
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049 2013/06/30)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.854	2.010
High resolution limit [Å]	1.900	1.900
Rmerge	0.099
Number of reflections	42415	6637
<I/σ(I)>	13.4
Completeness [%]	99.7	97.9
Redundancy	6.55	6.34
CC(1/2)	0.999	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M Bis-Tris pH 6.5, 2 M Ammonium sulphate.