6T62
Crystal structure of Acinetobacter baumannii FabG in complex with NADPH at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 87.647, 87.647, 151.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.820 - 1.800 |
| R-factor | 0.1631 |
| Rwork | 0.162 |
| R-free | 0.18750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4afn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.348 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.820 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.890 |
| Rmeas | 0.071 | |
| Rpim | 0.034 | 0.498 |
| Number of reflections | 51520 | 3200 |
| <I/σ(I)> | 18.6 | 2.2 |
| Completeness [%] | 93.7 | 95.2 |
| Redundancy | 8 | 7.7 |
| CC(1/2) | 0.999 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 2.1M DL-Malic acid pH7 |
