6T3T

Structure of the 4-hydroxy-tetrahydrodipicolinate synthase from the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	P 42
Unit cell lengths	109.060, 109.060, 107.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.773 - 2.100
R-factor	0.1836
Rwork	0.181
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yxg
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.773	2.150
High resolution limit [Å]	2.100	2.100
Rmeas	0.156	0.739
Number of reflections	74317	5359
<I/σ(I)>	9.2	2.4
Completeness [%]	99.8
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	273	0.05 M citric acid, 0.1 M lithium sulfate, 0.05 M sodium phosphate dibasic dihydrate, 19% w/v PEG 1000