6T3J
Dual Epitope Targeting by Anti-DR5 Antibodies
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.604, 90.926, 138.379 |
| Unit cell angles | 90.00, 103.79, 90.00 |
Refinement procedure
| Resolution | 134.410 - 3.050 |
| R-factor | 0.2508 |
| Rwork | 0.251 |
| R-free | 0.28190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.341 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 134.410 | 3.300 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.088 | 0.820 |
| Rmeas | 0.107 | 0.024 |
| Number of reflections | 55388 | 11644 |
| <I/σ(I)> | 10.69 | 1.77 |
| Completeness [%] | 98.5 | 99 |
| Redundancy | 3 | 3.1 |
| CC(1/2) | 0.997 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 281 | 10% (w/v) PEG 4000 200 mM ammonium sulphate 100 mM Sodium acetate pH 4.6 |
