6T3F

Crystal structure Nipah virus fusion glycoprotein in complex with a neutralising Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	P 63 2 2
Unit cell lengths	149.249, 149.249, 385.392
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	91.116 - 3.200
R-factor	0.2145
Rwork	0.213
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5evm 4jo1
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (dev_3488)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.300	3.250
High resolution limit [Å]	3.200	3.200
Rmerge	0.211	2.231
Rmeas	0.215	2.279
Rpim	0.042	0.456
Number of reflections	42722	2104
<I/σ(I)>	13.9	1.9
Completeness [%]	99.6	99.2
Redundancy	25.8	24.3
CC(1/2)	0.998	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.094 M Tris pH 8.0 and 3.7 M NaCl