6T2N
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.465, 96.128, 128.756 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.716 - 2.700 |
| Rwork | 0.233 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wut |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.202 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 33933 | 4096 |
| <I/σ(I)> | 7.7 | 1 |
| Completeness [%] | 99.7 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.991 | 0.485 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.6 M Tri sodium citrate pH 6.5 |
