6T2A

Mopeia Virus Exonuclease domain partially complexed with Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2018-06-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9720
Spacegroup name	P 1 2 1
Unit cell lengths	45.597, 37.969, 137.379
Unit cell angles	90.00, 93.31, 90.00

Refinement procedure

Resolution	43.969 - 2.000
R-factor	0.2345
Rwork	0.232
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sx8
Data reduction software	XDS (20190315)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	137.151	1.788
High resolution limit [Å]	1.758	1.758
Rmerge	0.029	0.361
Rpim	0.019	0.238
Number of reflections	44701	2273
<I/σ(I)>	17.1	2.1
Completeness [%]	94.3
Redundancy	3
CC(1/2)	0.999	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	0.1 M MES pH 6.5 and 25 % (M/W) PEG 8000)