6T1T
Cytochrome P450 reductase in complex with NADPH from Candida tropicalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2018-08-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.657, 67.497, 143.994 |
| Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
| Resolution | 74.740 - 2.080 |
| R-factor | 0.2215 |
| Rwork | 0.219 |
| R-free | 0.26720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bn4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.474 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.1) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 143.940 | 2.190 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.192 | 1.943 |
| Rmeas | 0.231 | 2.339 |
| Rpim | 0.127 | 1.286 |
| Total number of observations | 252423 | |
| Number of reflections | 82779 | 11409 |
| <I/σ(I)> | 4 | |
| Completeness [%] | 96.1 | 91.1 |
| Redundancy | 3 | 2.9 |
| CC(1/2) | 0.986 | 0.394 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 289 | 0.2 M Sodium malonate pH 5.0, 20% PEG3350 |
