6T1S
PPAR mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.660, 60.660, 169.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.330 - 1.650 |
| R-factor | 0.1726 |
| Rwork | 0.171 |
| R-free | 0.20440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xld |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.151 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 1.705 |
| High resolution limit [Å] | 1.646 | 1.646 |
| Rmerge | 0.010 | |
| Rpim | 0.010 | |
| Number of reflections | 39355 | 3818 |
| <I/σ(I)> | 25.75 | 0.95 |
| Completeness [%] | 99.7 | |
| Redundancy | 2 | |
| CC(1/2) | 1.000 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 25% PEG3350, 0.2 M LiSO4, BisTris 0.1M pH 6.5 |
