6T0Q

Pleurotus Ostreatus Lectin (POL), apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-01-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 61 2 2
Unit cell lengths	153.070, 153.070, 145.811
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.771 - 2.050
R-factor	0.1726
Rwork	0.172
R-free	0.18660
Structure solution method	MIR
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	SHARP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	132.562	63.882	2.160
High resolution limit [Å]	2.050	6.480	2.050
Rmerge		0.030	0.380
Rmeas	0.071	0.032	0.395
Rpim	0.020	0.010	0.107
Total number of observations	790176	24968	119417
Number of reflections	63266	2243	9074
<I/σ(I)>	25.9	54.1	7.4
Completeness [%]	99.8	99.4	99.7
Redundancy	12.5	11.1	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	Hepes 0.1 M pH 7.5, 0.8 M K/Na tartrate tetrahydrate