6SZQ
Crystal structure of human DDAH-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99985 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.146, 76.153, 116.839 |
| Unit cell angles | 90.00, 95.47, 90.00 |
Refinement procedure
| Resolution | 46.646 - 2.412 |
| R-factor | 0.2246 |
| Rwork | 0.223 |
| R-free | 0.27020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i2e |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.646 | 46.646 | 2.470 |
| High resolution limit [Å] | 2.410 | 10.790 | 2.410 |
| Rmerge | 0.118 | 0.037 | 1.011 |
| Rmeas | 0.130 | 0.041 | 1.109 |
| Number of reflections | 59837 | 704 | 4203 |
| <I/σ(I)> | 9.89 | 22.76 | 1.8 |
| Completeness [%] | 99.1 | 97.6 | 95.1 |
| Redundancy | 5.913 | 5.776 | 5.778 |
| CC(1/2) | 0.998 | 0.999 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.15 | 0.2M NaAc, 0.1M Tris pH 8,5, 30% PEG4000, cryo: reservoir+20% glycerol |
