6SY0
Structure of the Plasmodium falciparum SIP2 DNA-binding AP2 tandem repeat in complex with two SPE2 half-sites
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-08 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.91741 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 80.350, 43.720, 153.710 |
Unit cell angles | 90.00, 92.17, 90.00 |
Refinement procedure
Resolution | 43.931 - 3.102 |
R-factor | 0.2758 |
Rwork | 0.274 |
R-free | 0.31840 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 153.600 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.084 | 1.088 |
Rmeas | 0.091 | 1.164 |
Number of reflections | 19852 | 1912 |
<I/σ(I)> | 15.24 | 2.26 |
Completeness [%] | 97.0 | 100 |
Redundancy | 7.6 | 8 |
CC(1/2) | 1.000 | 0.927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 277 | 0.16 M calcium acetate 100 mM cacodylic acid 6.5% w/v PEG 8000 20% v/v glycerol |