6SXW
Crystal structure of the first RRM domain of human Zinc finger protein 638 (ZNF638)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 57.435, 57.435, 46.619 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.800 - 2.751 |
| R-factor | 0.2649 |
| Rwork | 0.260 |
| R-free | 0.31310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cq1 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.800 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.050 | 1.666 |
| Rpim | 0.018 | 0.569 |
| Number of reflections | 2464 | 343 |
| <I/σ(I)> | 20.9 | 1.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 9.4 | 9.5 |
| CC(1/2) | 1.000 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M tris pH 8.5 -- 2.6M ammonium sulfate |
