6SX7

Crystal Structure of the Voltage-Gated Sodium Channel NavMs (F208L) (2.2 Angstrom resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	I 4 2 2
Unit cell lengths	109.004, 109.004, 208.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.250 - 2.500
R-factor	0.232
Rwork	0.232
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hvx
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.250	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	22217	2459
<I/σ(I)>	15.1	2.8
Completeness [%]	100.0
Redundancy	16.4
CC(1/2)	0.998	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	0.1 M NaCl, 0.1 M Li2SO4, 0.1 M sodium citrate buffer, pH 5, 300% v/v polyethylene glycol (PEG) 300