6SX2
dsRNA recognition by R38AK41A mutant of H7N1 NS1 RNA Binding Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-20 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.690, 102.038, 102.696 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.796 - 1.900 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sw8 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.870 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.046 | 1.250 |
| Rmeas | 0.052 | 1.385 |
| Rpim | 0.023 | 0.591 |
| Total number of observations | 179050 | |
| Number of reflections | 34557 | 2202 |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.2 | 5.4 |
| CC(1/2) | 0.999 | 0.481 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 100mM sodium phosphate/citric acid, 200mM ammonium sulfate, 40% ethylene glycol |
