6SX2

dsRNA recognition by R38AK41A mutant of H7N1 NS1 RNA Binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 21 21 21
Unit cell lengths	40.690, 102.038, 102.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.796 - 1.900
R-factor	0.169
Rwork	0.168
R-free	0.19150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sw8
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.870	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.046	1.250
Rmeas	0.052	1.385
Rpim	0.023	0.591
Total number of observations	179050
Number of reflections	34557	2202
<I/σ(I)>	16.3
Completeness [%]	99.9	100
Redundancy	5.2	5.4
CC(1/2)	0.999	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	100mM sodium phosphate/citric acid, 200mM ammonium sulfate, 40% ethylene glycol