6SX0

Specific dsRNA recognition by wild type H7N1 NS1 RNA-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-08
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.98010
Spacegroup name	P 21 21 21
Unit cell lengths	49.785, 60.397, 87.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.259 - 1.750
R-factor	0.1731
Rwork	0.171
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sw8
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.260	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.052	1.546
Rmeas	0.057	1.675
Rpim	0.022	0.640
Number of reflections	27291	1481
<I/σ(I)>	16.7
Completeness [%]	99.9	100
Redundancy	6.5	6.8
CC(1/2)	0.999	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	100mM CHES, 30% PEG400