6SWS
The DBB dimerization domain of B-cell adaptor for PI3K (BCAP) is required for down regulation of inflammatory signalling through the Toll-like receptor pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2016-12-09 |
| Detector | DECTRIS PILATUS3 100K-M |
| Wavelength(s) | 0.97, 0.98 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 87.169, 87.169, 234.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.960 - 3.000 |
| R-factor | 0.1984 |
| Rwork | 0.194 |
| R-free | 0.23820 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.568 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.17rc4_3626) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.077 | 0.900 |
| Number of reflections | 155144 | 15856 |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 8.3 | 8.7 |
| CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 1.3 M NaH2PO4, 0.7M K2HPO4,0.1M sodium acetate pH4.5 |
