6SWP

C-TERMINAL BROMODOMAIN OF HUMAN BRD2 WITH iBET-BD2 (GSK046)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-28
Detector	RIGAKU
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	72.201, 52.390, 32.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.320 - 1.604
Rwork	0.154
R-free	0.19320
Structure solution method	FOURIER SYNTHESIS
RMSD bond angle	1.183
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.320	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	15434	1590
<I/σ(I)>	43.4	15.7
Completeness [%]	93.4
Redundancy	2.8
CC(1/2)	0.998	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	30% PEG 300, 0.1M MES buffer pH6.