6SWH

Crystal structure of the ternary complex between the type 1 pilus proteins FimC, FimI and FimA from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9
Spacegroup name	P 61
Unit cell lengths	198.350, 198.350, 87.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.891 - 2.800
R-factor	0.1713
Rwork	0.171
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sqb
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15rc3_3435)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.000	22.000	3.000
High resolution limit [Å]	2.800	21.000	2.800
Rmerge	0.066	0.024	0.576
Rmeas	0.072	0.027	0.643
Number of reflections	48258	18	8945
<I/σ(I)>	21.27	63.06	3.07
Completeness [%]	99.3	13.5	99.2
Redundancy	5.851	5.5	5.137

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.8 M sodium formate, 100 mM Tris/acetic acid pH 8.5, 16% PEG 4000 (w/v)