6SWG

Crystal structure of the TASOR-Periphilin core complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 32 2 1
Unit cell lengths	93.570, 93.570, 84.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.983 - 2.510
R-factor	0.2303
Rwork	0.228
R-free	0.27060
Structure solution method	SAD
Data reduction software	XDS (version 11)
Data scaling software	Aimless (version 0.5.32)
Phasing software	CRANK2 (version 2.0.148)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000	2.580
High resolution limit [Å]	2.510	2.510
Rmerge	0.085	1.087
Rmeas	0.089	1.146
Rpim	0.029	0.360
Number of reflections	15081	1113
<I/σ(I)>	15
Completeness [%]	99.9	100
Redundancy	10	10
CC(1/2)	0.998	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1M ammonium sulfate, 100 mM sodium citrate; protein buffer: PBS