6SWB
The REC domain of AraT, a response regulator from Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.499, 41.918, 43.981 |
| Unit cell angles | 83.46, 89.98, 71.11 |
Refinement procedure
| Resolution | 31.162 - 2.259 |
| R-factor | 0.1762 |
| Rwork | 0.168 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6swf |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.259 | 2.259 |
| Rmerge | 0.060 | 0.265 |
| Number of reflections | 9678 | 1203 |
| <I/σ(I)> | 17.3 | 5.2 |
| Completeness [%] | 94.5 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 41% PPG 425 and 0.1 M Bis-Tris pH 6 |
