6SW8

Crystal structure of the NS1 (H7N1) RNA-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-29
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.98009
Spacegroup name	P 31 2 1
Unit cell lengths	83.827, 83.827, 68.527
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.832 - 1.933
R-factor	0.1763
Rwork	0.175
R-free	0.19320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h5n
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.832	1.970
High resolution limit [Å]	1.930	1.930
Rmerge	0.078	1.090
Number of reflections	21170	21170
<I/σ(I)>	15.9	1.6
Completeness [%]	100.0
Redundancy	11.3
CC(1/2)	0.999	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.12M (Diethylene glycol, Triethylene glycol, Tetraethylene glycol, Pentaethylene glycol) + 0.1M (Sodium HEPES, MOPS) + 37.5% (MPD, PEG 1000, PEG 3350)