6SUJ

Mutations in SsgB correlate to longitudinal cell division during sporulation of Streptomyces coelicolor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.975
Spacegroup name	P 21 21 21
Unit cell lengths	67.933, 93.219, 96.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.167 - 3.200
Rwork	0.236
R-free	0.29350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6slc
RMSD bond length	0.007
RMSD bond angle	1.467
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.900	2.610
High resolution limit [Å]	2.560	2.560
Rmerge	0.446	3.642
Rmeas	0.466	3.894
Rpim	0.134	1.344
Number of reflections	20149	20149
<I/σ(I)>	4.8	1.1
Completeness [%]	99.6	91.6
Redundancy	12.1	8.4
CC(1/2)	0.991	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	0.2 M Potassium sodium tartrate, 0.1 M Bis-tris prop (pH 7.5), 20% PEG3350