6SUJ
Mutations in SsgB correlate to longitudinal cell division during sporulation of Streptomyces coelicolor
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.933, 93.219, 96.241 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.167 - 3.200 |
| Rwork | 0.236 |
| R-free | 0.29350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6slc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.467 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.900 | 2.610 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.446 | 3.642 |
| Rmeas | 0.466 | 3.894 |
| Rpim | 0.134 | 1.344 |
| Number of reflections | 20149 | 20149 |
| <I/σ(I)> | 4.8 | 1.1 |
| Completeness [%] | 99.6 | 91.6 |
| Redundancy | 12.1 | 8.4 |
| CC(1/2) | 0.991 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 0.2 M Potassium sodium tartrate, 0.1 M Bis-tris prop (pH 7.5), 20% PEG3350 |
