6SUC
Human PTPRU D1 domain, oxidised form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.090, 107.920, 89.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.190 - 1.970 |
| R-factor | 0.2154 |
| Rwork | 0.214 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sub |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.863 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.280 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.128 | 1.061 |
| Rpim | 0.055 | 0.458 |
| Number of reflections | 21600 | 1071 |
| <I/σ(I)> | 9 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 6.3 |
| CC(1/2) | 0.995 | 0.527 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M BICINE, pH 9, 1 M lithium chloride, 20% (w/v) polyethylene glycol 6000 |
