6SU1
Trypanosoma congolense pyruvate kinase in complex with citrate and glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-06-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 83.450, 107.150, 148.000 |
| Unit cell angles | 109.74, 90.51, 106.78 |
Refinement procedure
| Resolution | 49.330 - 3.000 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hyv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.108 | 0.843 |
| Rmeas | 0.147 | 1.146 |
| Rpim | 0.098 | 0.771 |
| Number of reflections | 85798 | 8940 |
| <I/σ(I)> | 6.2 | 0.91 |
| Completeness [%] | 93.4 | |
| Redundancy | 2 | |
| CC(1/2) | 0.989 | 0.364 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM ammonium citrate dibasic, 12.5% PEG 3350 |
