6STE

Crystal structure of the tick chemokine-binding protein Evasin-4 (SG 3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97717
Spacegroup name	P 32 2 1
Unit cell lengths	68.214, 68.214, 181.531
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.175 - 1.790
R-factor	0.1808
Rwork	0.179
R-free	0.20960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6st4
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.070	1.850
High resolution limit [Å]	1.790	1.790
Rmerge	0.200	0.101
Number of reflections	47147	3398
<I/σ(I)>	7.1	3
Completeness [%]	100.0
Redundancy	19.4
CC(1/2)	0.988	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	15% glycerol, 25.5% PEG 4000, 0.17 M ammonium acetate, 85 mM acetate buffer pH 4.6