6SSO
EDN mutant L45H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979256 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.120, 52.470, 56.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.489 - 1.211 |
R-factor | 0.1355 |
Rwork | 0.135 |
R-free | 0.15120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gqv |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.490 | 1.270 |
High resolution limit [Å] | 1.210 | 1.210 |
Number of reflections | 38717 | 1897 |
<I/σ(I)> | 6.6 | |
Completeness [%] | 99.6 | |
Redundancy | 4.1 | |
CC(1/2) | 0.976 | 0.866 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 100 mM sodium cacodylate, pH 7 and 1.6 M sodium acetate |