6SRU

Structure of Ig-like V-type domian of mouse Programmed cell death 1 ligand 1 (PD-L1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978631
Spacegroup name	P 1 21 1
Unit cell lengths	73.920, 107.715, 79.531
Unit cell angles	90.00, 99.15, 90.00

Refinement procedure

Resolution	49.664 - 2.532
R-factor	0.2302
Rwork	0.228
R-free	0.27910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c3t
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.664	2.620
High resolution limit [Å]	2.530	2.530
Rmerge	0.061	0.826
Number of reflections	38622	3645
<I/σ(I)>	10.8
Completeness [%]	94.1
Redundancy	2.6
CC(1/2)	0.998	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium sulfate, 0.1 M Tris pH 7.5, 20% PEG 5000 MME