6SRR
Crystal structure of human SHIP2 catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.130, 62.120, 115.620 |
| Unit cell angles | 90.00, 92.38, 90.00 |
Refinement procedure
| Resolution | 36.491 - 2.450 |
| R-factor | 0.1889 |
| Rwork | 0.186 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a9c |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.994 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.300 | 42.300 | 2.510 |
| High resolution limit [Å] | 2.450 | 10.960 | 2.450 |
| Rmerge | 0.096 | 0.057 | 1.000 |
| Rmeas | 0.109 | 0.065 | 1.141 |
| Rpim | 0.052 | 0.032 | 0.541 |
| Total number of observations | 103280 | 1062 | 7569 |
| Number of reflections | 23610 | 276 | 1733 |
| <I/σ(I)> | 9.5 | 24.2 | 1.7 |
| Completeness [%] | 99.5 | 96 | 99.8 |
| Redundancy | 4.4 | 3.8 | 4.4 |
| CC(1/2) | 0.996 | 0.997 | 0.505 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M Sodium citrate, 20 % w/v PEG 3000 |
