6SQW
Mouse dCTPase in complex with 5-Me-dCMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.516, 58.516, 140.905 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.040 - 1.800 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.596 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.500 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.130 | 1.360 |
| Number of reflections | 23533 | 32921 |
| <I/σ(I)> | 12.2 | 1.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.9 | |
| CC(1/2) | 0.990 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris pH 8.75, 2.4 M ammonium sulfate |
