6SQH
Crystal structure of mouse PRMT6 with partial C-terminal TEV cleavage site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-05 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.143, 118.106, 71.760 |
| Unit cell angles | 90.00, 103.24, 90.00 |
Refinement procedure
| Resolution | 36.851 - 2.390 |
| R-factor | 0.2116 |
| Rwork | 0.209 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c03 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3546) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.102 | 0.691 |
| Rmeas | 0.117 | 0.800 |
| Rpim | 0.056 | 0.396 |
| Number of reflections | 26129 | 2472 |
| <I/σ(I)> | 13.1 | 2 |
| Completeness [%] | 98.8 | 89.2 |
| Redundancy | 4.1 | 3.7 |
| CC(1/2) | 0.996 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG Smear Broad 22%, MES pH 6.5 100 mM |
