6SQ3
Crystal structure of mouse PRMT6 in complex with inhibitor U1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-07 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.526, 117.979, 71.945 |
| Unit cell angles | 90.00, 103.30, 90.00 |
Refinement procedure
| Resolution | 29.174 - 2.150 |
| R-factor | 0.2528 |
| Rwork | 0.251 |
| R-free | 0.29550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 4c03 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.110 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.088 | 0.871 |
| Rmeas | 0.101 | 1.166 |
| Rpim | 0.049 | 0.766 |
| Number of reflections | 36561 | 3146 |
| <I/σ(I)> | 10.4 | 1 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 4 | 2.2 |
| CC(1/2) | 0.996 | 0.294 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG 3,350 20%, CH3COONa 200 mM, Tris-HCl pH 7.5 100 mM |
