6SPP
Structure of the Escherichia coli methionyl-tRNA synthetase variant VI298
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.510, 45.530, 86.310 |
| Unit cell angles | 90.00, 107.38, 90.00 |
Refinement procedure
| Resolution | 38.900 - 1.490 |
| R-factor | 0.136 |
| Rwork | 0.134 |
| R-free | 0.17670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qqt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.977 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.910 | 1.590 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Number of reflections | 93868 | 14735 |
| <I/σ(I)> | 11.2 | 1.2 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 4.7 | |
| CC(1/2) | 0.998 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 1.08 M ammonium citrate, 10 mM potassium phosphate, pH 7.0 |
