6SNQ
Crystal structures of human PGM1 isoform 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.262, 53.155, 76.825 |
| Unit cell angles | 90.00, 99.14, 90.00 |
Refinement procedure
| Resolution | 48.630 - 2.700 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.481 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.630 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 0.560 |
| Rmeas | 0.092 | 0.632 |
| Rpim | 0.043 | 0.286 |
| Number of reflections | 16247 | 2160 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 99.6 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.997 | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | KCl, MgCl2, Na cacodylate pH 6.5 and PEG4000 |
