6SNL
(R)-selective amine transaminase from Exophiala sideris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 115.113, 301.521, 140.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.440 - 3.129 |
| R-factor | 0.2631 |
| Rwork | 0.262 |
| R-free | 0.28920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ce5 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.440 | 3.241 |
| High resolution limit [Å] | 3.129 | 3.129 |
| Rpim | 0.141 | 0.687 |
| Number of reflections | 43551 | 4277 |
| <I/σ(I)> | 5.22 | 1.08 |
| Completeness [%] | 99.0 | 97.36 |
| Redundancy | 13.1 | 13 |
| CC(1/2) | 0.978 | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | NaCl, Bis-Tris, PEG 3350 |
