6SMV
Structure of HPV49 E6 protein in complex with MAML1 LxxLL motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.906, 44.539, 94.237 |
| Unit cell angles | 90.00, 99.70, 90.00 |
Refinement procedure
| Resolution | 46.440 - 2.140 |
| R-factor | 0.2197 |
| Rwork | 0.218 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4giz |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.435 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc1_3769) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.440 | 2.220 |
| High resolution limit [Å] | 2.140 | 2.144 |
| Number of reflections | 63547 | 10215 |
| <I/σ(I)> | 11.14 | |
| Completeness [%] | 97.7 | |
| Redundancy | 7.6 | |
| CC(1/2) | 0.998 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.8 | 277 | Lithium Acetate 200mM, PEG 3350 22.5% |
