6SM2
Mutant immunoglobulin light chain causing amyloidosis (Pat-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-31 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 127.320, 127.320, 81.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.22710 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.247 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.055 | 0.496 |
| Number of reflections | 13924 | 1510 |
| <I/σ(I)> | 20.6 | 4.2 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 8.3 | |
| CC(1/2) | 0.999 | 0.962 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1 M sodiumcitrate, 0.5 M (NH4)2SO4, 1.0 M Li2SO4 |
