6SLC
Mutations in SsgB correlate to longitudinal cell division during sporulation of Streptomyces coelicolor
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.975 |
| Spacegroup name | I 4 |
| Unit cell lengths | 155.471, 155.471, 53.753 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.213 - 2.300 |
| Rwork | 0.208 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cm1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.413 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.927 | 2.309 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.065 | 1.009 |
| Rmeas | 0.076 | 1.167 |
| Rpim | 0.037 | 0.570 |
| Number of reflections | 33694 | 1579 |
| <I/σ(I)> | 9.4 | 1.1 |
| Completeness [%] | 95.5 | 92.1 |
| Redundancy | 4 | 4.1 |
| CC(1/2) | 0.979 | 0.477 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M Sodium chloride, 0.1 M Sodium/potassium phosphate (pH 6.2), 50% PEG200 |
